![]() All blots were washed identically using 2X SSC/0.1% SDS and 0.1X SSC/0.1% SDS. The blots were incubated in the hybridization buffers indicated, following the manufacturer's recommendations for time and temperature. Six identical Northern blots were assayed for p53 using 106 cpm/ml of a random-primed, labeled probe. ULTRAhyb™ Detects a Signal in Only 8 Hours, Compared to a Minimum of 5 Days Using Competitors' Hybridization Solutions. Single copy genes are readily detected in only 1 µg of genomic DNA by Southerns only compared to the 10 µg typically required when using standard hybridization buffers.įigure 1. Using ULTRAhyb with Northerns, mRNAs too rare to detect using standard means are detected in an overnight exposure. The benefits of greater sensitivity are remarkable. ULTRAhyb™, is as much as 100 times more sensitive than commonly used hybridization buffers (Figure 1). In many cases, maximizing the sensitivity of blot hybridization would forestall the need to switch to PCR-based detection methods, which are more difficult to set up and potentially less quantitative.Īmbion has developed a hybridization buffer that maximizes hybridization efficiency without increasing non-specific background. This limitation reduces the sensitivities of blot-associated assays by 20–100-fold, greatly reducing the ability to detect rare messages with Northern blots and dot blots, or to analyze limited DNA samples by Southern blotting. ![]() This is consistent with published work (1). Research at Ambion indicates that under typical blot hybridization conditions, only 0.5-5% of the target molecules on a membrane are actually bound by available probe. A primary limitation of all blot hybridizations is the efficiency of hybridization between the nucleic acids on the membrane, and the labeled nucleic acids in the hybridization solution. ![]()
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![]() ![]() The more I talked about the couch, the more I heard from people having the same problem. But I was obsessed, and all I could talk about was the couch. The craziest fucking couch in the world is still not more exciting than the Q train running on the R line because of scheduled track maintenance. You know how you’re not supposed to talk about the weather or your commute because they’re boring? The same is true of couches. ![]() I became obsessed with the extremely banal mistake I had made as a consumer. One direction was to “Hold the cushion properly and make sure the pointed end of the stick is all the way through, until you can see both ends of the stick on each side of the cushion.” I tried in earnest to follow the directions, but the wooden dowel would not fit into the buttonholes, and the entire exercise left me with fewer buttons than I started with. The kit was backordered, so it arrived two full months later and contained a wooden dowel, two buttons, and some directions that didn’t make sense. They sent me a button-repair kit, indicating that this probably happens a lot. I emailed customer service and asked if this was normal. As soon as one button had fallen off of our couch, it was like a spigot had been turned, allowing all of the other buttons to fall off, too. We would lean back slightly too far, and all of the cushions would shift forward and over the edge of the couch in unison. We would scooch across a cushion at the wrong angle, and a button would pop off, leaving a fraying hole behind. We sat on the couch at the end of each day and congratulated ourselves on our good and prudent choice and searched for throw pillows that didn’t have any words or foxes on them.Īround when the throw pillows finally arrived, the couch began to disintegrate in small ways. It was a little uncomfortable, but probably just needed some throw pillows to soften it. ![]() The couch came, and our old one, a vintage leather Craigslist number, left. If you buy a couch together, either the couch or the relationship will break, and the two things will have no correlation. Engagement rings are the biggest racket in history, and even if you love each other, one of you will lose your ring. No matter what Apple commercials and jewelry ads tell you, you should never, ever view an object as a metaphor for your relationship. This is the moment when I need to warn you of something vitally important. I looked at the image on the West Elm website and saw an entire montage of us laughing on the couch with friends, reading the Sunday paper on the couch, drinking obscure liqueurs on the couch (would this be the couch on which we would discover that we loved Cynar or Chartreuse?), moving the couch into a larger apartment, covering the couch with tarps while we painted the walls around it a daring color, giving birth on the couch, dying on the couch. It was more than we were used to paying for a piece of furniture, but the price seemed to be proof of enduring quality. We would each put a fat $600 towards the couch, and that money would be an investment into our new life together. We chose a West Elm design called the “Peggy” in a deep rusty orange color. The couch would be the most prominent piece of furniture in our small apartment and our first big purchase together - a gigantic spongy representation of our shared style sensibility. This is why, a few weeks after moving in with my partner, Kevin, we decided to buy a couch from West Elm. And more specifically, a need to prove that I’ve graduated from Walmart bedframes and second-hand plywood shelves scooped up from the sidewalk. The only couch anybody needs is a metal frame pulled from the curb, a few pillow cases, and a stack of old newspapers.īut in spite of myself, as a 28-year-old, I find myself drawn into the same capitalistic pitfall that many young professionals are drawn into - a need to prove my adulthood with mid-century furniture. Poly-Fil? Foam? Goose feathers? Forget about it. Every couch I have sat on since then has felt unreasonably, needlessly luxurious. When I was a kid my grandma had a couch on her front porch that was, as a result of some sort of thrifty post-wartime craft project, stuffed with crumpled-up newspapers. Comparing notes with other unsatisfied owners of the Peggy sofa ![]() ![]() ![]() Patients will usually apply them for 3 to 4 months in combination with benzoyl peroxide or a retinoid 11.Īfter this time, your healthcare provider may recommend you stop clindamycin but continue using benzoyl peroxide or retinoids. Healthcare providers usually intend for antibiotics like clindamycin to be used as shorter-term treatments. Stopping too early can increase your risk for antibiotic resistance. Antibiotics should always complete a full course to ensure the medication kills the bacteria. What Are Side Effects of Clindamycin Phosphate skin burning or itching, dry skin, skin redness, oily skin, skin peeling, stomach upset, or abdominal. Patients should not discontinue the use of clindamycin if they start seeing results. Gastrointestinal upset in both dogs and cats has been reported, including vomiting, diarrhea, and occasionally bloody. The medication should be applied on the whole affected by acne, not just to the pimples themselves, as this will prevent new spots from appearing 10. ![]() Some formulations, like foams, may contain alcohol stinging or burning when applied to the skin.īefore applying a clindamycin gel or lotion, patients must wash their face thoroughly with warm water and soap, rinse well, and pat dry 9. You may experience some application site reactions as side effects while using Klindex Gel, but they normally fade away with time. application and should be applied exactly as recommended by the doctor. Clindamycin helps to inhibit the growth of acne-causing bacteria. If a healthcare provider prescribes topical clindamycin, they advise patients to apply it twice daily. It contains clindamycin as an active component. ![]() ![]() ![]() View the first screenshots at the gallery. Visit the settlers between each run and discover their stories.Help a settlement flourish, bringing with it colorful characters and permanent upgrades.Learn more about the Overgrowth and the deep storylines hidden within with each death and subsequent attempt.Discover and unlock a wide arsenal of weapons and items to create the perfect build for every run.Face hordes of enemies and gigantic bosses in frenetic third-person combat.Play single player or lead your team of Breakers through the Overgrowth in online cooperative play.Use your wall-dashing, hoverboard, glider and more for incredible freedom across landscapes.It will share threads, aesthetics, lore and other recognizable elements, but will be a new game driven by new designs. It is set in the universe of Hyper Light and is its own story. A vast, ever-changing world awaits with massive, open biomes and deep labyrinths. Hyper Light Breaker is neither a sequel nor a prequel to Hyper Light Drifter.A world in disarray, with mysteries to solve, vicious enemies to fight, and fully 3D environments to explore.Play alone or with friends to explore massive biomes, defeat brutal monsters, create new builds, survive the mysterious Crowns and overthrow the almighty Abyss King in this action roguelite adventure from the creators of Hyper Light Drifter. Players are going to love exploring the Hyper Light universe like never before.”Įnter the Overgrowth, a new land in the world of Hyper Light. ![]() Hyper Light Drifter has a passionate community of players who fell in love with the game’s unique presentation, and Hyper Light Breaker will elevate that with its stylish 3D world and unforgettable art style alone. Gearbox Publishing San Francisco president Yoon Im added, “As huge fans of Hyper Light Drifter, we are thrilled to partner with Heart Machine to help bring Hyper Light Breaker to life. Fans put their trust in us back then to deliver on the vision we can’t wait for our community to experience Hyper Light Breaker-it’s a new path and culmination of everything we have learned over the past eight years of development at Heart Machine.” Crowdfunding for the game exceeded our wildest expectations, leading to millions of players from around the world falling in love with Hyper Light Drifter. We feel deeply fortunate to be able to celebrate the franchise in this way. “The long journey from the dream project getting Kickstarted back in 2013 to now has been incredible. “It’s wild to think it’s been six years since we released Hyper Light Drifter,” said Heart Machine creative director and Hyper Light Drifter creator Alx Preston in a press release. It will launch first in Early Access in spring 2023. Gearbox Publishing and developer Heart Machine have announced Hyper Light Breaker, a new roguelite action adventure game set in the world of Hyper Light Drifter, for PC ( Steam). ![]() ![]() ![]() There are no mounts or anything, but there's the standard, fast travel option, and there's Luffy's iconic, arm swinging that's used a lot at certain points in the game, and there's a second fast travel alternative that I hardly ever used because it's hardly ever available. The traversal options in the game aren't bad at all, they're actually pretty decent. If you don't enjoy the music in this game, then you have pretty high standards :P Probably my biggest issue with One Piece Odyssey is it's traversal. And that's a fair opinion, but the soundtrack was composed by the guy behind the Dark Souls soundtrack, as well as the "Tales of." series, to mention a few. One of the negative reviews criticized the soundtrack for not being very good. The developers have paid great attention to detail, and the world looks gorgeous at times. I imagine some people might have an issue with the art style they went for, but I personally think it looks very good. I finished my completionist run in about 70 hours, with all optional content done, excluding the post-game content with higher difficulty bosses from the One Piece universe. I enjoyed playing through the game with a completionist run, but I also couldn't help but notice it's many, smaller issues since I did absolutely everything there is to do in the game. I finished my completionist run in about 70 hours, with all optional First of all, I imagine fans of One Piece will enjoy this game, regardless of it's shortcomings. ![]() … Expandįirst of all, I imagine fans of One Piece will enjoy this game, regardless of it's shortcomings. Again, as a One Piece fan I just have a stupid smile on my face while I play this game must buy. Overall, an easy turn based jrpg that doesn't do anything THAT bad, and innovates enough for a pretty fun time. It's got the classics, so it's hard to complain. No dubs, which is good, sub master race rise up. Oda wrote for the game, and you can tell. You'll enjoy the little details throughout. As a One Piece fan this game could be a 9/9.5, but trying to come at it without that lense. They do have a lot of systems, which, if you engage with you make the game easier (ff8 style) Characters are ON POINT. Gameplay, while innovative, is a little easy. As a One Piece fan this game could Refreshing to see turn based gameplay, I'm not a die hard Han and TB style games, but I think innovation within the system warrants celebration. ![]() They do have a lot of systems, which, if you engage with you make the game easier (ff8 style)Ĭharacters are ON POINT. But during their voyage they are caught in a storm and shipwrecked.Refreshing to see turn based gameplay, I'm not a die hard Han and TB style games, but I think innovation within the system warrants celebration. The famed pirate, Monkey.D.Luffy, better known as Straw Hat Luffy, and his crew the Straw Hat Pirates, are sailing across the New World in search of the next island and the next adventure that awaits them. In the meantime, you can read more about the game below via the PlayStation Store. If official patch notes are released by Bandai Namco, we’ll be sure to update this post as soon as we can. Made it so the gameplay clock stopped during sleep mode or when the device was on the HOME screen.Made balance adjustments to some Event Battles.The only patch notes that have been released so far come from the game’s PS4 update history. One Piece Odyssey Update 1.04 Patch Notes | One Piece Odyssey March 16 Patch Notes: Check out the One Piece Odyssey March 16 patch notes. Developer ILCA has released One Piece Odyssey update 1.04 today for all platforms. ![]() ![]() ![]() A System or Data volume that isn’t correctly firmlinked to its partner simply can’t function.Īs these firmlinks are created during the creation of the paired volumes, adding them later may not be possible. In Big Sur and later, System and Data volumes are joined together by special bi-directional firmlinks, which are crucial to their function. ![]() This can be used in a two-step process on a disk image made from the original volume, or directly.Ĭloning a general-purpose APFS volume should be relatively straightforward using asr, but when it comes to the volumes most users want to clone, the System or Data volume from a macOS boot volume group, this becomes more complex. Because APFS volumes can have snapshots and inaccessible directories, and in special circumstances aren’t free-standing from other volumes, there’s now only one tool capable of cloning volumes, Apple Software Restore, asr. Because it’s usually performed between two different storage units, it isn’t normally expected that contents will be stored at exactly the same locations, and in the past it has been used to defragment data and free space, for instance by cloning a volume to another disk, then cloning it back to the original.Ĭloning HFS+ is fairly straightforward, and has been longstanding practice which isn’t reliant on specific tools. Most faithful copying occurs when the source and destination storage use the same file system, and no network transfer is involved, but methods of copying should always preserve the data in the file.Ĭloning a volume or a whole disk is a special form of copying in which the aim is to create a perfect replica of the original, including a file system identical in every respect, with identical data and metadata. Some xattrs are never copied, others always, provided the destination supports some means of storing them, which varies between different file systems. ![]() Non-standard file metadata, particularly extended attributes (xattrs), are even less predictable in their behaviour. That could mean that a 20 MB original is copied as a 50 GB file full of empty space. ![]() Do the same from an APFS volume to HFS+, which doesn’t support the format, and the sparse file explodes to its full size. As that copy changes from its original, APFS will normally store changed data, until eventually the data of those two files are completely separate.Ĭopy a sparse file within the same APFS volume, or to another APFS volume, and it should remain in this special space-saving format. Much of this stems from the capabilities of different file systems, and it’s useful to consider some examples from APFS.Ĭopy a file within an APFS volume and you’ll normally not duplicate the data at all, but end up with a clone file, which temporarily shares the same data in storage. Although copying should always be faithful to the data in files, it’s less reliable when it comes to its metadata and the precise representation of the data. You can copy individual files from one folder to another, to a different storage medium, and between remote systems. This is the generic term, and applies widely to many different processes. This article explains how they differ, and their consequences. There’s a tendency to use the terms copy, clone and backup as if they mean essentially the same thing. When making copies or backups of our files, it’s vital to make clear distinctions between different methods. ![]() ![]() If you do not understand what is causing this behavior, please contact us here. If you promise to stop (by clicking the Agree button below), we'll unblock your connection for now, but we will immediately re-block it if we detect additional bad behavior. Overusing our search engine with a very large number of searches in a very short amount of time.Using a badly configured (or badly written) browser add-on for blocking content.Running a "scraper" or "downloader" program that either does not identify itself or uses fake headers to elude detection.Using a script or add-on that scans GameFAQs for box and screen images (such as an emulator front-end), while overloading our search engine.There is no official GameFAQs app, and we do not support nor have any contact with the makers of these unofficial apps. Continued use of these apps may cause your IP to be blocked indefinitely. This triggers our anti-spambot measures, which are designed to stop automated systems from flooding the site with traffic. ![]() Some unofficial phone apps appear to be using GameFAQs as a back-end, but they do not behave like a real web browser does.Using GameFAQs regularly with these browsers can cause temporary and even permanent IP blocks due to these additional requests. ![]() If you are using the Brave browser, or have installed the Ghostery add-on, these programs send extra traffic to our servers for every page on the site that you browse, then send that data back to a third party, essentially spying on your browsing habits.We strongly recommend you stop using this browser until this problem is corrected. The latest version of the Opera browser sends multiple invalid requests to our servers for every page you visit. ![]() The most common causes of this issue are: Your IP address has been temporarily blocked due to a large number of HTTP requests. ![]() ![]() We had an area we wanted to camp which were the campsites at the back of the campground #71 and up. ![]() Due to the marsh and water in the area it’s likely you may see moose either near or far away in the grasslands. Timber Creek is usually the area where you can see the most moose. Along the way we encountered Big Horn Sheep, Colorado’s state animal and one we have been wanting to see for quite some time, and moose. We weren’t in a hurry as we wanted to make sure to enjoy it and show our friend from out of town around. We made our way to RMNP around 12 and made our way through the park on a 4th of July weekend. Timber Creek campground is a first-come first serve. Logs were available that you can buy at the campsite which we had to buy because we didn’t bring our own thinking there was a. The fee was $20 per night and although there was a fire nearby a couple of weeks ago we were able to burn a fire. Despite it also being summer we didn’t hear a lot of kids running around. I will say despite the site being close to the entrance and the road our stay was pretty quiet. By the time we got to the campsite all those were taken and we had to settle for a site near the entrance. ![]() ![]() ![]() ![]() ![]() The word rest in “God Rest Ye Merry Gentlemen” simply means keep or make. This is due to another word that has a much different meaning in today’s world and a lost punctuation mark. Ye means you, but even when translated to “God rest you mighty gentlemen,” the song still makes very little sense. So when the English carolers of the Victorian era sang, “merry gentlemen,” they meant great or mighty men. Thus, in the Middle Ages, a strong army was a merry army, a great singer was a merry singer, and a mighty ruler was a merry ruler. Robin Hood’s “Merry Men” might have been happy, but the merry that described them meant great and mighty. When “God Rest Ye Merry Gentlemen” was written, merry had a very different meaning. But that's the men for you.When modern people say “Merry” Christmas, the word merry means happy. I told him I wasn't in the habit of fertilizing my back yard with cream. He upsot a churnful of cream in the back yard-and was just as cool as a cowcumber over it-laughed and said it was good for the land. ![]() I put up with him till harvest was in, and then one day my patience give out. When it is encountered in literature, it is usually as part of character speech: Upsot is a rare dialectical past participial form of upset that was probably chosen for its rhyme with "lot." Despite its use in "Jingle Bells," upsot is entered in very few dictionaries. ![]() The lesser-sung later verses are ripe with unusually dark narrative, including the sleigh becoming overturned and the narrator's wistful advice, voiced in the imperative, to "go it while you're young." It has a long tradition as a staple of schoolchildren, who are often taught nothing beyond the innocuous first verse. "Jingle Bells" is one of the most popular holiday tunes, written in 1850 by James Lord Pierpont, the uncle of financier J. The word's Christmas pedigree precedes its use in song however it occurs in Luke 2:10 of the King James Bible (in a line made popular to many generations of children by Linus Van Pelt), when the angel delivers the news of the arrival of the Savior: "Fear not: for behold, I bring you good tidings of great joy, which shall be to all people." Although there is nothing specific in the meaning or origin of tiding that pertains to Christmas (it derives via Middle English from Old English and relates to betide, meaning "to happen especially by fate"), we most often see the word in contexts pertaining to the Christmas season. Tiding is defined as a piece of news, and is often found in the plural, modified by good or glad. Tidings appears in a number of Christmas carols in addition to "We Wish You a Merry Christmas," we also find it in "God Rest Ye Merry, Gentlemen," which speaks of "tidings of comfort and joy." We wish you a merry Christmas and a happy new year Good tidings we bring to you and your kin With that interpretation, "gentlemen" becomes an instance of direct address: "be well and happy, Gentlemen." But "god rest you merry" was, at the time the text was written, an established expression of good wishes in its own right: The first line of this very old (15th century, by some estimates) song has a number of elements that typically throw listeners for a loop in addition to the ambiguity of the verb rest, there's the swapping out of "you" for the ecclesiastical pronoun "ye" in some versions, as well as inconsistency with regard to where the comma is placed.īecause some texts place the comma before "merry," some readers or listeners interpret the adjective as modifying "gentlemen," leaving rest as a transitive verb with you as its object (for a phrasing similar to "God rest your soul" or "God bless you"). ![]() ![]() ![]() If you’re looking for something a little more kick, our own Sriracha sauce is an excellent choice. Hot Ones’ Last Dab (The Last Dab Reduxx and The Last Dab XXX), which begins in Season 6, is unquestionably the most intense sauce on the menu, and it’s guaranteed to leave a fiery taste in your mouth. Buffalo wings are a zesty delight, but hot wings will make your mouth water. The spiciness of each sauce is especially important, as the two sauces differ greatly. As of season 4, Hot Ones’ own The Last Dab (The Last Dab Reduxx) and The Last Dab XXX (Season 10) have Scoville ratings of 2,000,000+. As the first sauce, Sriracha has a Scoville rating of 2,200 and is relatively mild. Hot Wings, on the other hand, are made of spicy ingredients and come in a variety of flavors. ![]() However, in general, these wings tend to be on the milder side when it comes to spice levels.īuffalo wings are an American classic, and they are typically served with a mild sauce such as Sriracha. There is no one-size-fits-all answer to this question, as the level of spiciness in Pizza Hut buffalo wings can vary depending on the location and the cook. If you dare, go ahead and order yourself some Buffalo Burnin’ Hot wings and see if you can handle them. “Buffalo Burnin'” is a song about a Buffalo What is hot? When you lick the Buffalo Burnin’ Hot sauce from Pizza Hut’s chicken wings, you will inhale its fumes. Chicken wings are glazed and shiny in a hot sauce and spices marinade that makes them juicy, oily, and sticky. They are so deep-fried that they are impossible to eat. But be warned, these wings are not for the faint of heart.īuffalo wings at Pizza Hut are juicy, spicy, and delectable. These wings are so hot, in fact, that they come with a disclaimer: “Please use caution when consuming.” If you’re looking for a challenge and enjoy spicy food, then you’ll want to try Pizza Hut’s Buffalo Burnin’ Hot Wings. Pizza Hut’s Buffalo Burnin’ Hot Wings are some of the spiciest wings on the market. ![]() |
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